Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture

Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by e...

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Autores principales: Abbah, Sunny A., Thomas, Dilip, Browne, Shane, O’Brien, Timothy, Pandit, Abhay, Zeugolis, Dimitrios I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748261/
https://www.ncbi.nlm.nih.gov/pubmed/26860065
http://dx.doi.org/10.1038/srep20922
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author Abbah, Sunny A.
Thomas, Dilip
Browne, Shane
O’Brien, Timothy
Pandit, Abhay
Zeugolis, Dimitrios I.
author_facet Abbah, Sunny A.
Thomas, Dilip
Browne, Shane
O’Brien, Timothy
Pandit, Abhay
Zeugolis, Dimitrios I.
author_sort Abbah, Sunny A.
collection PubMed
description Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-β1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-β1 and a range of pro-fibrotic extracellular matrix genes.
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spelling pubmed-47482612016-02-17 Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture Abbah, Sunny A. Thomas, Dilip Browne, Shane O’Brien, Timothy Pandit, Abhay Zeugolis, Dimitrios I. Sci Rep Article Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-β1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-β1 and a range of pro-fibrotic extracellular matrix genes. Nature Publishing Group 2016-02-10 /pmc/articles/PMC4748261/ /pubmed/26860065 http://dx.doi.org/10.1038/srep20922 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Abbah, Sunny A.
Thomas, Dilip
Browne, Shane
O’Brien, Timothy
Pandit, Abhay
Zeugolis, Dimitrios I.
Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
title Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
title_full Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
title_fullStr Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
title_full_unstemmed Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
title_short Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
title_sort co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748261/
https://www.ncbi.nlm.nih.gov/pubmed/26860065
http://dx.doi.org/10.1038/srep20922
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