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Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG
The genetically encoded photosensitizer miniSOG (mini Singlet Oxygen Generator) can be used to kill cells in C. elegans. miniSOG generates the reactive oxygen species (ROS) singlet oxygen after illumination with blue light. Illumination of neurons expressing miniSOG targeted to the outer mitochondri...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748272/ https://www.ncbi.nlm.nih.gov/pubmed/26861262 http://dx.doi.org/10.1038/srep21271 |
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author | Xu, Suhong Chisholm, Andrew D. |
author_facet | Xu, Suhong Chisholm, Andrew D. |
author_sort | Xu, Suhong |
collection | PubMed |
description | The genetically encoded photosensitizer miniSOG (mini Singlet Oxygen Generator) can be used to kill cells in C. elegans. miniSOG generates the reactive oxygen species (ROS) singlet oxygen after illumination with blue light. Illumination of neurons expressing miniSOG targeted to the outer mitochondrial membrane (mito-miniSOG) causes neuronal death. To enhance miniSOG’s efficiency as an ablation tool in multiple cell types we tested alternative targeting signals. We find that membrane targeted miniSOG allows highly efficient cell killing. When combined with a point mutation that increases miniSOG’s ROS generation, membrane targeted miniSOG can ablate neurons in less than one tenth the time of mito-miniSOG. We extend the miniSOG ablation technique to non-neuronal tissues, revealing an essential role for the epidermis in locomotion. These improvements expand the utility and throughput of optogenetic cell ablation in C. elegans. |
format | Online Article Text |
id | pubmed-4748272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47482722016-02-17 Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG Xu, Suhong Chisholm, Andrew D. Sci Rep Article The genetically encoded photosensitizer miniSOG (mini Singlet Oxygen Generator) can be used to kill cells in C. elegans. miniSOG generates the reactive oxygen species (ROS) singlet oxygen after illumination with blue light. Illumination of neurons expressing miniSOG targeted to the outer mitochondrial membrane (mito-miniSOG) causes neuronal death. To enhance miniSOG’s efficiency as an ablation tool in multiple cell types we tested alternative targeting signals. We find that membrane targeted miniSOG allows highly efficient cell killing. When combined with a point mutation that increases miniSOG’s ROS generation, membrane targeted miniSOG can ablate neurons in less than one tenth the time of mito-miniSOG. We extend the miniSOG ablation technique to non-neuronal tissues, revealing an essential role for the epidermis in locomotion. These improvements expand the utility and throughput of optogenetic cell ablation in C. elegans. Nature Publishing Group 2016-02-10 /pmc/articles/PMC4748272/ /pubmed/26861262 http://dx.doi.org/10.1038/srep21271 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xu, Suhong Chisholm, Andrew D. Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG |
title | Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG |
title_full | Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG |
title_fullStr | Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG |
title_full_unstemmed | Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG |
title_short | Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG |
title_sort | highly efficient optogenetic cell ablation in c. elegans using membrane-targeted minisog |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748272/ https://www.ncbi.nlm.nih.gov/pubmed/26861262 http://dx.doi.org/10.1038/srep21271 |
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