A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

Electron microscopy-based connectomics aims to comprehensively map synaptic connections in neural tissue. However, current approaches are limited in their capacity to directly assign molecular identities to neurons. Here, we use serial multiplex immunogold labeling (siGOLD) and serial-section transm...

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Detalles Bibliográficos
Autores principales: Shahidi, Réza, Williams, Elizabeth A, Conzelmann, Markus, Asadulina, Albina, Verasztó, Csaba, Jasek, Sanja, Bezares-Calderón, Luis A, Jékely, Gáspár
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749568/
https://www.ncbi.nlm.nih.gov/pubmed/26670546
http://dx.doi.org/10.7554/eLife.11147

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749568/
https://www.ncbi.nlm.nih.gov/pubmed/26670546
http://dx.doi.org/10.7554/eLife.11147

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