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Efficient, Low-Cost Nucleofection of Passaged Chondrocytes

Nucleofection of chondrocytes has been shown to be an adequate method of transfection. Using Amaxa’s nucleofection system, transfection efficiencies up to 89% were achievable for vector (pmaxGFP) and 98% for siRNA (siGLO) into passaged chondrocytes. However, such methods rely on costly commercial ki...

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Detalles Bibliográficos
Autores principales: Parreno, Justin, Delve, Elizabeth, Andrejevic, Katarina, Paez-Parent, Sabrina, Wu, Po-han, Kandel, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749754/
https://www.ncbi.nlm.nih.gov/pubmed/26958320
http://dx.doi.org/10.1177/1947603515609399