Cargando…
Efficient, Low-Cost Nucleofection of Passaged Chondrocytes
Nucleofection of chondrocytes has been shown to be an adequate method of transfection. Using Amaxa’s nucleofection system, transfection efficiencies up to 89% were achievable for vector (pmaxGFP) and 98% for siRNA (siGLO) into passaged chondrocytes. However, such methods rely on costly commercial ki...
Autores principales: | Parreno, Justin, Delve, Elizabeth, Andrejevic, Katarina, Paez-Parent, Sabrina, Wu, Po-han, Kandel, Rita |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4749754/ https://www.ncbi.nlm.nih.gov/pubmed/26958320 http://dx.doi.org/10.1177/1947603515609399 |
Ejemplares similares
-
Superficial and deep zone articular chondrocytes exhibit differences in actin polymerization status and actin-associated molecules in vitro
por: Delve, Elizabeth, et al.
Publicado: (2020) -
High Efficiency Low Cost Fibroblast Nucleofection for GMP Compatible Cell-based Gene Therapy
por: Zhang, Ziyang, et al.
Publicado: (2017) -
Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
por: Maeß, Marten B., et al.
Publicado: (2014) -
Nucleofection: A New Method for Cutaneous Gene Transfer?
por: Jacobsen, Frank, et al.
Publicado: (2006) -
Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection
por: Bradburne, Christopher, et al.
Publicado: (2009)