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Intracellular and Extracellular Expression of Bacillus thuringiensis Crystal Protein Cry5B in Lactococcus lactis for Use as an Anthelminthic

The Bacillus thuringiensis crystal (Cry) protein Cry5B (140 kDa) and a truncated version of the protein, tCry5B (79 kDa), are lethal to nematodes. Genes encoding the two proteins were separately cloned into a high-copy-number vector with a strong constitutive promoter (pTRK593) in Lactococcus lactis...

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Detalles Bibliográficos
Autores principales: Durmaz, Evelyn, Hu, Yan, Aroian, Raffi V., Klaenhammer, Todd R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751831/
https://www.ncbi.nlm.nih.gov/pubmed/26682852
http://dx.doi.org/10.1128/AEM.02365-15