Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity

Using a Systematic Evolution of Ligands by Exponential Enrichment (SELEX) protocol capable of selecting xeno-nucleic acid (XNA) aptamers, a 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamer (referred to as FA1) to HIV-1 reverse transcriptase (HIV-1 RT) was selected. FA1 bound HIV-1 RT with K(D,app)...

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Detalles Bibliográficos
Autores principales: Alves Ferreira-Bravo, Irani, Cozens, Christopher, Holliger, Philipp, DeStefano, Jeffrey J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751925/
https://www.ncbi.nlm.nih.gov/pubmed/26476448
http://dx.doi.org/10.1093/nar/gkv1057

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4751925/
https://www.ncbi.nlm.nih.gov/pubmed/26476448
http://dx.doi.org/10.1093/nar/gkv1057

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