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An efficient protocol for isolation of inhibitor-free nucleic acids even from recalcitrant plants

For fast and easy isolation of inhibitor-free genomic DNA even from the toughest plant leaf samples, including those high in polyphenols and polysaccharides, a protocol has been developed. To prevent the solubility of polysaccharides in the DNA extract, high salt concentration (1.4 M) was used in th...

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Detalles Bibliográficos
Autores principales: Rezadoost, Mohammad Hossein, Kordrostami, Mojtaba, Kumleh, Hassan Hassani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752943/
https://www.ncbi.nlm.nih.gov/pubmed/28330131
http://dx.doi.org/10.1007/s13205-016-0375-0