Overexpression of Soluble Recombinant Human Lysyl Oxidase by Using Solubility Tags: Effects on Activity and Solubility

Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus...

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Detalles Bibliográficos
Autores principales: Smith, Madison A., Gonzalez, Jesica, Hussain, Anjum, Oldfield, Rachel N., Johnston, Kathryn A., Lopez, Karlo M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753049/
https://www.ncbi.nlm.nih.gov/pubmed/26942005
http://dx.doi.org/10.1155/2016/5098985

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753049/
https://www.ncbi.nlm.nih.gov/pubmed/26942005
http://dx.doi.org/10.1155/2016/5098985

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