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Overexpression of Soluble Recombinant Human Lysyl Oxidase by Using Solubility Tags: Effects on Activity and Solubility
Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus...
Autores principales: | Smith, Madison A., Gonzalez, Jesica, Hussain, Anjum, Oldfield, Rachel N., Johnston, Kathryn A., Lopez, Karlo M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753049/ https://www.ncbi.nlm.nih.gov/pubmed/26942005 http://dx.doi.org/10.1155/2016/5098985 |
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