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A CAPS-based binding assay provides semi-quantitative validation of protein-DNA interactions

Investigation of protein-DNA interactions provides crucial information for understanding the mechanisms of gene regulation. Current methods for studying protein-DNA interactions, such as DNaseI footprinting or gel shift assays, involve labeling DNA with radioactive or fluorescent tags, making these...

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Detalles Bibliográficos
Autores principales: Xie, Yongyao, Zhang, Yaling, Zhao, Xiucai, Liu, Yao-Guang, Chen, Letian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753479/
https://www.ncbi.nlm.nih.gov/pubmed/26877240
http://dx.doi.org/10.1038/srep21030