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Characterising protein/detergent complexes by triple-detection size-exclusion chromatography

BACKGROUND: In vitro investigations of membrane proteins usually depend on detergents for protein solubilisation and stabilisation. The amount of detergent bound to a membrane protein is relevant to successful experiment design and data analysis but is often unknown. Triple-detection size-exclusion...

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Detalles Bibliográficos
Autores principales: Gimpl, Katharina, Klement, Jessica, Keller, Sandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753644/
https://www.ncbi.nlm.nih.gov/pubmed/26880869
http://dx.doi.org/10.1186/s12575-015-0031-9