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Characterising protein/detergent complexes by triple-detection size-exclusion chromatography
BACKGROUND: In vitro investigations of membrane proteins usually depend on detergents for protein solubilisation and stabilisation. The amount of detergent bound to a membrane protein is relevant to successful experiment design and data analysis but is often unknown. Triple-detection size-exclusion...
Autores principales: | Gimpl, Katharina, Klement, Jessica, Keller, Sandro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753644/ https://www.ncbi.nlm.nih.gov/pubmed/26880869 http://dx.doi.org/10.1186/s12575-015-0031-9 |
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