Cargando…
Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.)
The uptake of glycine by pakchoi (Brassica chinensis L.), when supplied as single N-source or in a mixture of glycine and inorganic N, was studied at different light intensities under sterile conditions. At the optimal intensity (414 μmol m(−2) s(−1)) for plant growth, glycine, nitrate, and ammonium...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756379/ https://www.ncbi.nlm.nih.gov/pubmed/26882864 http://dx.doi.org/10.1038/srep21200 |
_version_ | 1782416323099230208 |
---|---|
author | Ma, Qingxu Cao, Xiaochuang Wu, Lianghuan Mi, Wenhai Feng, Ying |
author_facet | Ma, Qingxu Cao, Xiaochuang Wu, Lianghuan Mi, Wenhai Feng, Ying |
author_sort | Ma, Qingxu |
collection | PubMed |
description | The uptake of glycine by pakchoi (Brassica chinensis L.), when supplied as single N-source or in a mixture of glycine and inorganic N, was studied at different light intensities under sterile conditions. At the optimal intensity (414 μmol m(−2) s(−1)) for plant growth, glycine, nitrate, and ammonium contributed 29.4%, 39.5%, and 31.1% shoot N, respectively, and light intensity altered the preferential absorption of N sources. The lower (15)N-nitrate in root but higher in shoot and the higher (15)N-glycine in root but lower in shoot suggested that most (15)N-nitrate uptake by root transported to shoot rapidly, with the shoot being important for nitrate assimilation, and the N contribution of glycine was limited by post-uptake metabolism. The amount of glycine that was taken up by the plant was likely limited by root uptake at low light intensities and by the metabolism of ammonium produced by glycine at high light intensities. These results indicate that pakchoi has the ability to uptake a large quantity of glycine, but that uptake is strongly regulated by light intensity, with metabolism in the root inhibiting its N contribution. |
format | Online Article Text |
id | pubmed-4756379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47563792016-02-25 Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) Ma, Qingxu Cao, Xiaochuang Wu, Lianghuan Mi, Wenhai Feng, Ying Sci Rep Article The uptake of glycine by pakchoi (Brassica chinensis L.), when supplied as single N-source or in a mixture of glycine and inorganic N, was studied at different light intensities under sterile conditions. At the optimal intensity (414 μmol m(−2) s(−1)) for plant growth, glycine, nitrate, and ammonium contributed 29.4%, 39.5%, and 31.1% shoot N, respectively, and light intensity altered the preferential absorption of N sources. The lower (15)N-nitrate in root but higher in shoot and the higher (15)N-glycine in root but lower in shoot suggested that most (15)N-nitrate uptake by root transported to shoot rapidly, with the shoot being important for nitrate assimilation, and the N contribution of glycine was limited by post-uptake metabolism. The amount of glycine that was taken up by the plant was likely limited by root uptake at low light intensities and by the metabolism of ammonium produced by glycine at high light intensities. These results indicate that pakchoi has the ability to uptake a large quantity of glycine, but that uptake is strongly regulated by light intensity, with metabolism in the root inhibiting its N contribution. Nature Publishing Group 2016-02-17 /pmc/articles/PMC4756379/ /pubmed/26882864 http://dx.doi.org/10.1038/srep21200 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ma, Qingxu Cao, Xiaochuang Wu, Lianghuan Mi, Wenhai Feng, Ying Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) |
title | Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) |
title_full | Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) |
title_fullStr | Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) |
title_full_unstemmed | Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) |
title_short | Light intensity affects the uptake and metabolism of glycine by pakchoi (Brassica chinensis L.) |
title_sort | light intensity affects the uptake and metabolism of glycine by pakchoi (brassica chinensis l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756379/ https://www.ncbi.nlm.nih.gov/pubmed/26882864 http://dx.doi.org/10.1038/srep21200 |
work_keys_str_mv | AT maqingxu lightintensityaffectstheuptakeandmetabolismofglycinebypakchoibrassicachinensisl AT caoxiaochuang lightintensityaffectstheuptakeandmetabolismofglycinebypakchoibrassicachinensisl AT wulianghuan lightintensityaffectstheuptakeandmetabolismofglycinebypakchoibrassicachinensisl AT miwenhai lightintensityaffectstheuptakeandmetabolismofglycinebypakchoibrassicachinensisl AT fengying lightintensityaffectstheuptakeandmetabolismofglycinebypakchoibrassicachinensisl |