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In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures

Here, a method for presenting crystals of soluble and membrane proteins growing in the lipid cubic or sponge phase for in situ diffraction data collection at cryogenic temperatures is introduced. The method dispenses with the need for the technically demanding and inefficient crystal-harvesting step...

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Detalles Bibliográficos
Autores principales: Huang, Chia-Ying, Olieric, Vincent, Ma, Pikyee, Howe, Nicole, Vogeley, Lutz, Liu, Xiangyu, Warshamanage, Rangana, Weinert, Tobias, Panepucci, Ezequiel, Kobilka, Brian, Diederichs, Kay, Wang, Meitian, Caffrey, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756617/
https://www.ncbi.nlm.nih.gov/pubmed/26894538
http://dx.doi.org/10.1107/S2059798315021683