Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene

The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel–Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3A > T). The ICM was isolated using laser mi...

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Autores principales: Miere, Cristian, Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab resource: Stem cell line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757726/
https://www.ncbi.nlm.nih.gov/pubmed/27345783
http://dx.doi.org/10.1016/j.scr.2015.12.003
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author Miere, Cristian
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Miere, Cristian
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Miere, Cristian
collection PubMed
description The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel–Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3A > T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
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spelling pubmed-47577262016-03-02 Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene Miere, Cristian Hewitson, Heema Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab resource: Stem cell line The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel–Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3A > T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-01 /pmc/articles/PMC4757726/ /pubmed/27345783 http://dx.doi.org/10.1016/j.scr.2015.12.003 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Lab resource: Stem cell line
Miere, Cristian
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene
title Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_full Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_fullStr Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_full_unstemmed Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_short Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene
title_sort generation of kcl016 research grade human embryonic stem cell line carrying a mutation in vhl gene
topic Lab resource: Stem cell line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757726/
https://www.ncbi.nlm.nih.gov/pubmed/27345783
http://dx.doi.org/10.1016/j.scr.2015.12.003
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