A Modular Assembly Platform for Rapid Generation of DNA Constructs

Traditional cloning methods have limitations on the number of DNA fragments that can be simultaneously manipulated, which dramatically slows the pace of molecular assembly. Here we describe GMAP, a Gibson assembly-based modular assembly platform consisting of a collection of promoters and genes, whi...

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Detalles Bibliográficos
Autores principales: Akama-Garren, Elliot H., Joshi, Nikhil S., Tammela, Tuomas, Chang, Gregory P., Wagner, Bethany L., Lee, Da-Yae, Rideout III, William M., Papagiannakopoulos, Thales, Xue, Wen, Jacks, Tyler
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757859/
https://www.ncbi.nlm.nih.gov/pubmed/26887506
http://dx.doi.org/10.1038/srep16836

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757859/
https://www.ncbi.nlm.nih.gov/pubmed/26887506
http://dx.doi.org/10.1038/srep16836

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