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A Modular Assembly Platform for Rapid Generation of DNA Constructs
Traditional cloning methods have limitations on the number of DNA fragments that can be simultaneously manipulated, which dramatically slows the pace of molecular assembly. Here we describe GMAP, a Gibson assembly-based modular assembly platform consisting of a collection of promoters and genes, whi...
Autores principales: | Akama-Garren, Elliot H., Joshi, Nikhil S., Tammela, Tuomas, Chang, Gregory P., Wagner, Bethany L., Lee, Da-Yae, Rideout III, William M., Papagiannakopoulos, Thales, Xue, Wen, Jacks, Tyler |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757859/ https://www.ncbi.nlm.nih.gov/pubmed/26887506 http://dx.doi.org/10.1038/srep16836 |
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