Residue proximity information and protein model discrimination using saturation-suppressor mutagenesis

Identification of residue-residue contacts from primary sequence can be used to guide protein structure prediction. Using Escherichia coli CcdB as the test case, we describe an experimental method termed saturation-suppressor mutagenesis to acquire residue contact information. In this methodology, f...

Descripción completa

Detalles Bibliográficos
Autores principales: Sahoo, Anusmita, Khare, Shruti, Devanarayanan, Sivasankar, Jain, Pankaj C., Varadarajan, Raghavan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Biophysics and Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758949/
https://www.ncbi.nlm.nih.gov/pubmed/26716404
http://dx.doi.org/10.7554/eLife.09532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758949/
https://www.ncbi.nlm.nih.gov/pubmed/26716404
http://dx.doi.org/10.7554/eLife.09532

Ejemplares similares