Purification of Active Respiratory Supercomplex from Bovine Heart Mitochondria Enables Functional Studies

To understand the roles of mitochondrial respiratory chain supercomplexes, methods for consistently separating and preparing supercomplexes must be established. To this end, we solubilized supercomplexes from bovine heart mitochondria with digitonin and then replaced digitonin with amphipol (A8–35),...

Descripción completa

Detalles Bibliográficos
Autores principales: Shinzawa-Itoh, Kyoko, Shimomura, Harunobu, Yanagisawa, Sachiko, Shimada, Satoru, Takahashi, Ryoko, Oosaki, Marika, Ogura, Takashi, Tsukihara, Tomitake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2016
Materias:
Bioenergetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759192/
https://www.ncbi.nlm.nih.gov/pubmed/26698328
http://dx.doi.org/10.1074/jbc.M115.680553

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759192/
https://www.ncbi.nlm.nih.gov/pubmed/26698328
http://dx.doi.org/10.1074/jbc.M115.680553

Ejemplares similares