Cargando…

Purification of Active Respiratory Supercomplex from Bovine Heart Mitochondria Enables Functional Studies

To understand the roles of mitochondrial respiratory chain supercomplexes, methods for consistently separating and preparing supercomplexes must be established. To this end, we solubilized supercomplexes from bovine heart mitochondria with digitonin and then replaced digitonin with amphipol (A8–35),...

Descripción completa

Detalles Bibliográficos
Autores principales:	Shinzawa-Itoh, Kyoko, Shimomura, Harunobu, Yanagisawa, Sachiko, Shimada, Satoru, Takahashi, Ryoko, Oosaki, Marika, Ogura, Takashi, Tsukihara, Tomitake
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2016
Materias:	Bioenergetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759192/ https://www.ncbi.nlm.nih.gov/pubmed/26698328 http://dx.doi.org/10.1074/jbc.M115.680553

Ejemplares similares

X-ray structural analyses of azide-bound cytochrome c oxidases reveal that the H-pathway is critically important for the proton-pumping activity
por: Shimada, Atsuhiro, et al.
Publicado: (2018)

Isolation and Characterization of a Hybrid Respiratory Supercomplex Consisting of Mycobacterium tuberculosis Cytochrome bcc and Mycobacterium smegmatis Cytochrome aa(3)*
por: Kim, Mi-Sun, et al.
Publicado: (2015)

Structure of bovine cytochrome c oxidase in the ligand-free reduced state at neutral pH
por: Luo, Fangjia, et al.
Publicado: (2018)

Structure of bovine cytochrome c oxidase crystallized at a neutral pH using a fluorinated detergent
por: Luo, Fangjia, et al.
Publicado: (2017)

Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly
por: Hinchy, Elizabeth C., et al.
Publicado: (2018)

Modulation of Energy Transfer Pathways between Mitochondria and Myofibrils by Changes in Performance of Perfused Heart
por: Vendelin, Marko, et al.
Publicado: (2010)

The Deactive Form of Respiratory Complex I from Mammalian Mitochondria Is a Na(+)/H(+) Antiporter
por: Roberts, Philippa G., et al.
Publicado: (2012)

Inorganic polyphosphate is produced and hydrolyzed in F(0)F(1)-ATP synthase of mammalian mitochondria
por: Baev, Artyom Y., et al.
Publicado: (2020)

Two-dimensional crystallization of monomeric bovine cytochrome c oxidase with bound cytochrome c in reconstituted lipid membranes
por: Osuda, Yukiho, et al.
Publicado: (2016)

Complex I within Oxidatively Stressed Bovine Heart Mitochondria Is Glutathionylated on Cys-531 and Cys-704 of the 75-kDa Subunit: POTENTIAL ROLE OF CYS RESIDUES IN DECREASING OXIDATIVE DAMAGE
por: Hurd, Thomas R., et al.
Publicado: (2008)

ChChd3, an Inner Mitochondrial Membrane Protein, Is Essential for Maintaining Crista Integrity and Mitochondrial Function
por: Darshi, Manjula, et al.
Publicado: (2011)

Menaquinone-7 Is Specific Cofactor in Tetraheme Quinol Dehydrogenase CymA
por: McMillan, Duncan G. G., et al.
Publicado: (2012)

Molecular Dynamics Simulations of Creatine Kinase and Adenine Nucleotide Translocase in Mitochondrial Membrane Patch
por: Karo, Jaanus, et al.
Publicado: (2012)

A Pivotal Heme-transfer Reaction Intermediate in Cytochrome c Biogenesis
por: Mavridou, Despoina A. I., et al.
Publicado: (2012)

Superoxide Is Produced by the Reduced Flavin in Mitochondrial Complex I: A SINGLE, UNIFIED MECHANISM THAT APPLIES DURING BOTH FORWARD AND REVERSE ELECTRON TRANSFER
por: Pryde, Kenneth R., et al.
Publicado: (2011)

The Antisense RNA As1_flv4 in the Cyanobacterium Synechocystis sp. PCC 6803 Prevents Premature Expression of the flv4-2 Operon upon Shift in Inorganic Carbon Supply
por: Eisenhut, Marion, et al.
Publicado: (2012)

Reconstitution of Vacuolar-type Rotary H(+)-ATPase/Synthase from Thermus thermophilus
por: Kishikawa, Jun-ichi, et al.
Publicado: (2012)

Functional Characterization of drim2, the Drosophila melanogaster Homolog of the Yeast Mitochondrial Deoxynucleotide Transporter
por: Da-Rè, Caterina, et al.
Publicado: (2014)

Leigh Syndrome in Drosophila melanogaster: MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF Surf1 POST-TRANSCRIPTIONAL SILENCING
por: Da-Rè, Caterina, et al.
Publicado: (2014)

Targeting and Import Mechanism of Coiled-coil Helix Coiled-coil Helix Domain-containing Protein 3 (ChChd3) into the Mitochondrial Intermembrane Space
por: Darshi, Manjula, et al.
Publicado: (2012)

Electrostatic Ratchet in the Protective Antigen Channel Promotes Anthrax Toxin Translocation
por: Wynia-Smith, Sarah L., et al.
Publicado: (2012)

NdhM Subunit Is Required for the Stability and the Function of NAD(P)H Dehydrogenase Complexes Involved in CO(2) Uptake in Synechocystis sp. Strain PCC 6803
por: He, Zhihui, et al.
Publicado: (2016)

Tuning of Hemes b Equilibrium Redox Potential Is Not Required for Cross-Membrane Electron Transfer
por: Pintscher, Sebastian, et al.
Publicado: (2016)

Trends in Thermostability Provide Information on the Nature of Substrate, Inhibitor, and Lipid Interactions with Mitochondrial Carriers
por: Crichton, Paul G., et al.
Publicado: (2015)

Mitochondrial Disease-related Mutation G167P in Cytochrome b of Rhodobacter capsulatus Cytochrome bc(1) (S151P in Human) Affects the Equilibrium Distribution of [2Fe-2S] Cluster and Generation of Superoxide
por: Borek, Arkadiusz, et al.
Publicado: (2015)

Post-translational Modifications near the Quinone Binding Site of Mammalian Complex I
por: Carroll, Joe, et al.
Publicado: (2013)

Meclizine Inhibits Mitochondrial Respiration through Direct Targeting of Cytosolic Phosphoethanolamine Metabolism
por: Gohil, Vishal M., et al.
Publicado: (2013)

NDUFAF5 Hydroxylates NDUFS7 at an Early Stage in the Assembly of Human Complex I
por: Rhein, Virginie F., et al.
Publicado: (2016)

Lipid, Detergent, and Coomassie Blue G-250 Affect the Migration of Small Membrane Proteins in Blue Native Gels: MITOCHONDRIAL CARRIERS MIGRATE AS MONOMERS NOT DIMERS
por: Crichton, Paul G., et al.
Publicado: (2013)

Identification and quantification of protein S-nitrosation by nitrite in the mouse heart during ischemia
por: Chouchani, Edward T., et al.
Publicado: (2017)

Control of mitochondrial superoxide production by reverse electron transport at complex I
por: Robb, Ellen L., et al.
Publicado: (2018)

Oxidative stress alters mitochondrial bioenergetics and modifies pancreatic cell death independently of cyclophilin D, resulting in an apoptosis-to-necrosis shift
por: Armstrong, Jane A., et al.
Publicado: (2018)

Substitutions in the β subunits of sickle-cell hemoglobin improve oxidative stability and increase the delay time of sickle-cell fiber formation
por: Meng, Fantao, et al.
Publicado: (2019)

The CcmC–CcmE interaction during cytochrome c maturation by System I is driven by protein–protein and not protein–heme contacts
por: Shevket, Shevket H., et al.
Publicado: (2018)

Respiratory Complex I in Bos taurus and Paracoccus denitrificans Pumps Four Protons across the Membrane for Every NADH Oxidized
por: Jones, Andrew J. Y., et al.
Publicado: (2017)

The structure and reactivity of the HoxEFU complex from the cyanobacterium Synechocystis sp. PCC 6803
por: Artz, Jacob H., et al.
Publicado: (2020)

CLCA4 and MS4A12 as the significant gene biomarkers of primary colorectal cancer
por: Han, Jing, et al.
Publicado: (2020)

The effect of TGFβ1 on thermogenic markers is dependent on the degree of adipocyte differentiation
por: Maharjan, Babu R., et al.
Publicado: (2020)

The effect of group B streptococcus on maternal and infants’ prognosis in Guizhou, China
por: Dai, Wei, et al.
Publicado: (2019)

Crystal structure of a hemerythrin-like protein from Mycobacterium kansasii and homology model of the orthologous Rv2633c protein of M. tuberculosis
por: Ma, Zhongxin, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_roaek9lhn2nqpvmv8oq6c272hq