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rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
Osteoblast migration is significant in skeletal development. Recently, high mobility group box 1 protein (HMGB1) has been shown to highly expressed in cartilage to regulate endochondral ossification. Nevertheless, whether HMGB1 can modulate osteoblast proliferation and migration is poorly understood...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759610/ https://www.ncbi.nlm.nih.gov/pubmed/26744383 http://dx.doi.org/10.1042/BSR20150239 |
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author | Li, Ming-Jing Li, Fan Xu, Jian Liu, Yu-Dong Hu, Tao Chen, Jian-Ting |
author_facet | Li, Ming-Jing Li, Fan Xu, Jian Liu, Yu-Dong Hu, Tao Chen, Jian-Ting |
author_sort | Li, Ming-Jing |
collection | PubMed |
description | Osteoblast migration is significant in skeletal development. Recently, high mobility group box 1 protein (HMGB1) has been shown to highly expressed in cartilage to regulate endochondral ossification. Nevertheless, whether HMGB1 can modulate osteoblast proliferation and migration is poorly understood, as well as the intracellular signalling pathways that are involved in this process. Herein, we examined the effects of recombinant human HMGB1 (rhHMGB1) on the proliferation and migration of rat osteoblasts and investigated whether Toll-like receptor 2 (TLR2)- and TLR4-dependent signalling pathways are involved in the regulation of intracellular signalling. A transwell chamber assay was used to evaluate the migration of osteoblasts and the MTT assay was used to assess osteoblast proliferation. rhHMGB1 could significantly promote the migration of osteoblasts without inhibiting their proliferation. Meanwhile, rhHMGB1 can increase the nuclear translocation of nuclear factor-kappa B (NF-κB) p65. Specific siRNA constructs that target TLR2 or TLR4 could markedly inhibit HMGB1-induced migration of osteoblasts and HMGB1-enhanced activation of NF-κB. Collectively, HMGB1 could significantly enhance the migration of osteoblasts in vitro, and TLR2/TLR4-dependent NF-κB pathways are involved in HMGB1-induced osteoblast migration. |
format | Online Article Text |
id | pubmed-4759610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47596102016-03-03 rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner Li, Ming-Jing Li, Fan Xu, Jian Liu, Yu-Dong Hu, Tao Chen, Jian-Ting Biosci Rep Original Papers Osteoblast migration is significant in skeletal development. Recently, high mobility group box 1 protein (HMGB1) has been shown to highly expressed in cartilage to regulate endochondral ossification. Nevertheless, whether HMGB1 can modulate osteoblast proliferation and migration is poorly understood, as well as the intracellular signalling pathways that are involved in this process. Herein, we examined the effects of recombinant human HMGB1 (rhHMGB1) on the proliferation and migration of rat osteoblasts and investigated whether Toll-like receptor 2 (TLR2)- and TLR4-dependent signalling pathways are involved in the regulation of intracellular signalling. A transwell chamber assay was used to evaluate the migration of osteoblasts and the MTT assay was used to assess osteoblast proliferation. rhHMGB1 could significantly promote the migration of osteoblasts without inhibiting their proliferation. Meanwhile, rhHMGB1 can increase the nuclear translocation of nuclear factor-kappa B (NF-κB) p65. Specific siRNA constructs that target TLR2 or TLR4 could markedly inhibit HMGB1-induced migration of osteoblasts and HMGB1-enhanced activation of NF-κB. Collectively, HMGB1 could significantly enhance the migration of osteoblasts in vitro, and TLR2/TLR4-dependent NF-κB pathways are involved in HMGB1-induced osteoblast migration. Portland Press Ltd. 2016-02-19 /pmc/articles/PMC4759610/ /pubmed/26744383 http://dx.doi.org/10.1042/BSR20150239 Text en © 2016 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 (http://creativecommons.org/licenses/by/3.0/) . |
spellingShingle | Original Papers Li, Ming-Jing Li, Fan Xu, Jian Liu, Yu-Dong Hu, Tao Chen, Jian-Ting rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner |
title | rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner |
title_full | rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner |
title_fullStr | rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner |
title_full_unstemmed | rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner |
title_short | rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner |
title_sort | rhhmgb1 drives osteoblast migration in a tlr2/tlr4- and nf-κb-dependent manner |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759610/ https://www.ncbi.nlm.nih.gov/pubmed/26744383 http://dx.doi.org/10.1042/BSR20150239 |
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