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rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner

Osteoblast migration is significant in skeletal development. Recently, high mobility group box 1 protein (HMGB1) has been shown to highly expressed in cartilage to regulate endochondral ossification. Nevertheless, whether HMGB1 can modulate osteoblast proliferation and migration is poorly understood...

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Autores principales: Li, Ming-Jing, Li, Fan, Xu, Jian, Liu, Yu-Dong, Hu, Tao, Chen, Jian-Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759610/
https://www.ncbi.nlm.nih.gov/pubmed/26744383
http://dx.doi.org/10.1042/BSR20150239
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author Li, Ming-Jing
Li, Fan
Xu, Jian
Liu, Yu-Dong
Hu, Tao
Chen, Jian-Ting
author_facet Li, Ming-Jing
Li, Fan
Xu, Jian
Liu, Yu-Dong
Hu, Tao
Chen, Jian-Ting
author_sort Li, Ming-Jing
collection PubMed
description Osteoblast migration is significant in skeletal development. Recently, high mobility group box 1 protein (HMGB1) has been shown to highly expressed in cartilage to regulate endochondral ossification. Nevertheless, whether HMGB1 can modulate osteoblast proliferation and migration is poorly understood, as well as the intracellular signalling pathways that are involved in this process. Herein, we examined the effects of recombinant human HMGB1 (rhHMGB1) on the proliferation and migration of rat osteoblasts and investigated whether Toll-like receptor 2 (TLR2)- and TLR4-dependent signalling pathways are involved in the regulation of intracellular signalling. A transwell chamber assay was used to evaluate the migration of osteoblasts and the MTT assay was used to assess osteoblast proliferation. rhHMGB1 could significantly promote the migration of osteoblasts without inhibiting their proliferation. Meanwhile, rhHMGB1 can increase the nuclear translocation of nuclear factor-kappa B (NF-κB) p65. Specific siRNA constructs that target TLR2 or TLR4 could markedly inhibit HMGB1-induced migration of osteoblasts and HMGB1-enhanced activation of NF-κB. Collectively, HMGB1 could significantly enhance the migration of osteoblasts in vitro, and TLR2/TLR4-dependent NF-κB pathways are involved in HMGB1-induced osteoblast migration.
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spelling pubmed-47596102016-03-03 rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner Li, Ming-Jing Li, Fan Xu, Jian Liu, Yu-Dong Hu, Tao Chen, Jian-Ting Biosci Rep Original Papers Osteoblast migration is significant in skeletal development. Recently, high mobility group box 1 protein (HMGB1) has been shown to highly expressed in cartilage to regulate endochondral ossification. Nevertheless, whether HMGB1 can modulate osteoblast proliferation and migration is poorly understood, as well as the intracellular signalling pathways that are involved in this process. Herein, we examined the effects of recombinant human HMGB1 (rhHMGB1) on the proliferation and migration of rat osteoblasts and investigated whether Toll-like receptor 2 (TLR2)- and TLR4-dependent signalling pathways are involved in the regulation of intracellular signalling. A transwell chamber assay was used to evaluate the migration of osteoblasts and the MTT assay was used to assess osteoblast proliferation. rhHMGB1 could significantly promote the migration of osteoblasts without inhibiting their proliferation. Meanwhile, rhHMGB1 can increase the nuclear translocation of nuclear factor-kappa B (NF-κB) p65. Specific siRNA constructs that target TLR2 or TLR4 could markedly inhibit HMGB1-induced migration of osteoblasts and HMGB1-enhanced activation of NF-κB. Collectively, HMGB1 could significantly enhance the migration of osteoblasts in vitro, and TLR2/TLR4-dependent NF-κB pathways are involved in HMGB1-induced osteoblast migration. Portland Press Ltd. 2016-02-19 /pmc/articles/PMC4759610/ /pubmed/26744383 http://dx.doi.org/10.1042/BSR20150239 Text en © 2016 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Original Papers
Li, Ming-Jing
Li, Fan
Xu, Jian
Liu, Yu-Dong
Hu, Tao
Chen, Jian-Ting
rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
title rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
title_full rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
title_fullStr rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
title_full_unstemmed rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
title_short rhHMGB1 drives osteoblast migration in a TLR2/TLR4- and NF-κB-dependent manner
title_sort rhhmgb1 drives osteoblast migration in a tlr2/tlr4- and nf-κb-dependent manner
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759610/
https://www.ncbi.nlm.nih.gov/pubmed/26744383
http://dx.doi.org/10.1042/BSR20150239
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