Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

BACKGROUND: The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from Pseudomonas putida, various strategies for the over-expression of P14K are n...

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Detalles Bibliográficos
Autores principales: Sun, Weifeng, Zhu, Longbao, Chen, Xianggui, Chen, Ping, Yang, Lingling, Ding, Wenwu, Zhou, Zhemin, Liu, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761151/
https://www.ncbi.nlm.nih.gov/pubmed/26897378
http://dx.doi.org/10.1186/s12896-016-0252-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761151/
https://www.ncbi.nlm.nih.gov/pubmed/26897378
http://dx.doi.org/10.1186/s12896-016-0252-2

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