Biochemical Characterization of Kat1: a Domesticated hAT-Transposase that Induces DNA Hairpin Formation and MAT-Switching

Kluyveromyces lactis hAT-transposase 1 (Kat1) generates hairpin-capped DNA double strand breaks leading to MAT-switching (MATa to MATα). Using purified Kat1, we demonstrate the importance of terminal inverted repeats and subterminal repeats for its endonuclease activity. Kat1 promoted joining of the...

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Detalles Bibliográficos
Autores principales: Chiruvella, Kishore K., Rajaei, Naghmeh, Jonna, Venkateswara Rao, Hofer, Anders, Åström, Stefan U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763223/
https://www.ncbi.nlm.nih.gov/pubmed/26902909
http://dx.doi.org/10.1038/srep21671

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763223/
https://www.ncbi.nlm.nih.gov/pubmed/26902909
http://dx.doi.org/10.1038/srep21671

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