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Cloning and characterization of filamentous temperature-sensitive protein Z from Xanthomonas oryzae pv. Oryzae

The ftsZ gene from Xanthomonas oryzae pv. Oryzae was amplified by PCR with the specific primers, and the recombinant plasmid pET-22b-ftsZ was constructed successfully. The FtsZ with a 6× His tag was overexpressed in a soluble form in Escherichia coli BL21 and purified through a Ni-NTA agarose column...

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Detalles Bibliográficos
Autores principales: Dai, Leng, Huang, Yunhong, Chen, Yang, Long, Zhong-er
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764595/
https://www.ncbi.nlm.nih.gov/pubmed/27026842
http://dx.doi.org/10.1186/s40064-016-1876-3