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Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization

Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane prot...

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Detalles Bibliográficos
Autores principales: Hansen, Debra T., Robida, Mark D., Craciunescu, Felicia M., Loskutov, Andrey V., Dörner, Katerina, Rodenberry, John-Charles, Wang, Xiao, Olson, Tien L., Patel, Hetal, Fromme, Petra, Sykes, Kathryn F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764931/
https://www.ncbi.nlm.nih.gov/pubmed/26908053
http://dx.doi.org/10.1038/srep21925