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Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling
Porphyrins were attached to LNA uridine building blocks via rigid 5-acetylene or more flexible propargyl-amide linkers and incorporated into DNA strands. The systems show a greatly increased thermodynamic stability when using as little as three porphyrins in a zipper arrangement. Thermodynamic analy...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766578/ https://www.ncbi.nlm.nih.gov/pubmed/26416024 http://dx.doi.org/10.1039/c5ob01681a |
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author | Singleton, Daniel G. Hussain, Rohanah Siligardi, Giuliano Kumar, Pawan Hrdlicka, Patrick J. Berova, Nina Stulz, Eugen |
author_facet | Singleton, Daniel G. Hussain, Rohanah Siligardi, Giuliano Kumar, Pawan Hrdlicka, Patrick J. Berova, Nina Stulz, Eugen |
author_sort | Singleton, Daniel G. |
collection | PubMed |
description | Porphyrins were attached to LNA uridine building blocks via rigid 5-acetylene or more flexible propargyl-amide linkers and incorporated into DNA strands. The systems show a greatly increased thermodynamic stability when using as little as three porphyrins in a zipper arrangement. Thermodynamic analysis reveals clustering of the strands into more ordered duplexes with both greater negative ΔΔS and ΔΔH values, and less ordered duplexes with small positive ΔΔS differences, depending on the combination of linkers used. The exciton coupling between the porphyrins is dependent on the flanking DNA sequence in the single stranded form, and on the nature of the linker between the nucleobase and the porphyrin in the double stranded form; it is, however, also strongly influenced by intermolecular interactions. This system is suitable for the formation of stable helical chromophore arrays with sequence and structure dependent exciton coupling. |
format | Online Article Text |
id | pubmed-4766578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-47665782016-03-08 Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling Singleton, Daniel G. Hussain, Rohanah Siligardi, Giuliano Kumar, Pawan Hrdlicka, Patrick J. Berova, Nina Stulz, Eugen Org Biomol Chem Chemistry Porphyrins were attached to LNA uridine building blocks via rigid 5-acetylene or more flexible propargyl-amide linkers and incorporated into DNA strands. The systems show a greatly increased thermodynamic stability when using as little as three porphyrins in a zipper arrangement. Thermodynamic analysis reveals clustering of the strands into more ordered duplexes with both greater negative ΔΔS and ΔΔH values, and less ordered duplexes with small positive ΔΔS differences, depending on the combination of linkers used. The exciton coupling between the porphyrins is dependent on the flanking DNA sequence in the single stranded form, and on the nature of the linker between the nucleobase and the porphyrin in the double stranded form; it is, however, also strongly influenced by intermolecular interactions. This system is suitable for the formation of stable helical chromophore arrays with sequence and structure dependent exciton coupling. Royal Society of Chemistry 2016-01-07 2015-09-29 /pmc/articles/PMC4766578/ /pubmed/26416024 http://dx.doi.org/10.1039/c5ob01681a Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Singleton, Daniel G. Hussain, Rohanah Siligardi, Giuliano Kumar, Pawan Hrdlicka, Patrick J. Berova, Nina Stulz, Eugen Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling |
title | Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling
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title_full | Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling
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title_fullStr | Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling
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title_full_unstemmed | Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling
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title_short | Increased duplex stabilization in porphyrin-LNA zipper arrays with structure dependent exciton coupling
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title_sort | increased duplex stabilization in porphyrin-lna zipper arrays with structure dependent exciton coupling |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766578/ https://www.ncbi.nlm.nih.gov/pubmed/26416024 http://dx.doi.org/10.1039/c5ob01681a |
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