Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II)

BACKGROUND: The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic research and drug development. For example, antibody-drug conjugates represent a...

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Detalles Bibliográficos
Autores principales: York, Dona, Baker, Jeanne, Holder, Patrick G., Jones, Lesley C., Drake, Penelope M., Barfield, Robyn M., Bleck, Gregory T., Rabuka, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766608/
https://www.ncbi.nlm.nih.gov/pubmed/26911368
http://dx.doi.org/10.1186/s12896-016-0254-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766608/
https://www.ncbi.nlm.nih.gov/pubmed/26911368
http://dx.doi.org/10.1186/s12896-016-0254-0

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