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Comparison of fecal pooling methods and DNA extraction kits for the detection of Mycobacterium avium subspecies paratuberculosis
The aim of the study was to develop a sensitive method using quantitative real‐time polymerase chain reaction (qPCR) with pooled fecal samples for the screening of Johne's disease (JD). Manufacturer‐specified and our new pooling method in combination with five commercial kits for DNA extraction...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767428/ https://www.ncbi.nlm.nih.gov/pubmed/26666871 http://dx.doi.org/10.1002/mbo3.318 |