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Comparison of fecal pooling methods and DNA extraction kits for the detection of Mycobacterium avium subspecies paratuberculosis

The aim of the study was to develop a sensitive method using quantitative real‐time polymerase chain reaction (qPCR) with pooled fecal samples for the screening of Johne's disease (JD). Manufacturer‐specified and our new pooling method in combination with five commercial kits for DNA extraction...

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Detalles Bibliográficos
Autores principales:	Mita, Akiko, Mori, Yasuyuki, Nakagawa, Tetsuo, Tasaki, Tomoko, Utiyama, Katsuo, Mori, Hitomi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2015
Materias:	Original Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767428/ https://www.ncbi.nlm.nih.gov/pubmed/26666871 http://dx.doi.org/10.1002/mbo3.318

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