Enhancing the selective extracellular location of a recombinant E. coli domain antibody by management of fermentation conditions

The preparation of a recombinant protein using Escherichia coli often involves a challenging primary recovery sequence. This is due to the inability to secrete the protein to the extracellular space without a significant degree of cell lysis. This results in the release of nucleic acids, leading to...

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Detalles Bibliográficos
Autores principales: Voulgaris, Ioannis, Finka, Gary, Uden, Mark, Hoare, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Biotechnological Products and Process Engineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768232/
https://www.ncbi.nlm.nih.gov/pubmed/26184976
http://dx.doi.org/10.1007/s00253-015-6799-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768232/
https://www.ncbi.nlm.nih.gov/pubmed/26184976
http://dx.doi.org/10.1007/s00253-015-6799-3

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