Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects

Homologous recombination of single-stranded oligonucleotides is a highly efficient process for introducing precise mutations into the genome of E. coli and other organisms when mismatch repair (MMR) is disabled. This can result in the rapid accumulation of off-target mutations that can mask desired...

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Detalles Bibliográficos
Autores principales: Lennen, Rebecca M., Nilsson Wallin, Annika I., Pedersen, Margit, Bonde, Mads, Luo, Hao, Herrgård, Markus J., Sommer, Morten O. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770203/
https://www.ncbi.nlm.nih.gov/pubmed/26496947
http://dx.doi.org/10.1093/nar/gkv1090

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770203/
https://www.ncbi.nlm.nih.gov/pubmed/26496947
http://dx.doi.org/10.1093/nar/gkv1090

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