Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq

We present multiplex Digenome-seq to profile genome-wide specificities of up to 11 CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human genomic DNA was digested using multiple sgRNAs combined with the Cas9 protein and then subjected to whole-genome sequencing. In vitr...

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Detalles Bibliográficos
Autores principales: Kim, Daesik, Kim, Sojung, Kim, Sunghyun, Park, Jeongbin, Kim, Jin-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772022/
https://www.ncbi.nlm.nih.gov/pubmed/26786045
http://dx.doi.org/10.1101/gr.199588.115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772022/
https://www.ncbi.nlm.nih.gov/pubmed/26786045
http://dx.doi.org/10.1101/gr.199588.115

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