CRISPR/Cas9‐induced disruption of gene expression in mouse embryonic brain and single neural stem cells in vivo

We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into the embryonic neocortex of Tis21::GFP knock‐in mice, ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Kalebic, Nereo, Taverna, Elena, Tavano, Stefania, Wong, Fong Kuan, Suchold, Dana, Winkler, Sylke, Huttner, Wieland B, Sarov, Mihail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Scientific Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772980/
https://www.ncbi.nlm.nih.gov/pubmed/26758805
http://dx.doi.org/10.15252/embr.201541715

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772980/
https://www.ncbi.nlm.nih.gov/pubmed/26758805
http://dx.doi.org/10.15252/embr.201541715

Ejemplares similares