A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α

A novel dinuclear Ir(III) complex 5 was discovered to be specific to G-quadruplex DNA, and was utilized in a label-free G-quadruplex-based detection platform for transcription factor activity. The principle of this assay was demonstrated by using HIF-1α as a model protein. Moreover, this HIF-1α dete...

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Autores principales: Lu, Lihua, Wang, Modi, Mao, Zhifeng, Kang, Tian-Shu, Chen, Xiu-Ping, Lu, Jin-Jian, Leung, Chung-Hang, Ma, Dik-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773817/
https://www.ncbi.nlm.nih.gov/pubmed/26932240
http://dx.doi.org/10.1038/srep22458
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author Lu, Lihua
Wang, Modi
Mao, Zhifeng
Kang, Tian-Shu
Chen, Xiu-Ping
Lu, Jin-Jian
Leung, Chung-Hang
Ma, Dik-Lung
author_facet Lu, Lihua
Wang, Modi
Mao, Zhifeng
Kang, Tian-Shu
Chen, Xiu-Ping
Lu, Jin-Jian
Leung, Chung-Hang
Ma, Dik-Lung
author_sort Lu, Lihua
collection PubMed
description A novel dinuclear Ir(III) complex 5 was discovered to be specific to G-quadruplex DNA, and was utilized in a label-free G-quadruplex-based detection platform for transcription factor activity. The principle of this assay was demonstrated by using HIF-1α as a model protein. Moreover, this HIF-1α detection assay exhibited potential use for biological sample analysis.
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spelling pubmed-47738172016-03-09 A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α Lu, Lihua Wang, Modi Mao, Zhifeng Kang, Tian-Shu Chen, Xiu-Ping Lu, Jin-Jian Leung, Chung-Hang Ma, Dik-Lung Sci Rep Article A novel dinuclear Ir(III) complex 5 was discovered to be specific to G-quadruplex DNA, and was utilized in a label-free G-quadruplex-based detection platform for transcription factor activity. The principle of this assay was demonstrated by using HIF-1α as a model protein. Moreover, this HIF-1α detection assay exhibited potential use for biological sample analysis. Nature Publishing Group 2016-03-02 /pmc/articles/PMC4773817/ /pubmed/26932240 http://dx.doi.org/10.1038/srep22458 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lu, Lihua
Wang, Modi
Mao, Zhifeng
Kang, Tian-Shu
Chen, Xiu-Ping
Lu, Jin-Jian
Leung, Chung-Hang
Ma, Dik-Lung
A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α
title A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α
title_full A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α
title_fullStr A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α
title_full_unstemmed A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α
title_short A novel dinuclear iridium(III) complex as a G-quadruplex-selective probe for the luminescent switch-on detection of transcription factor HIF-1α
title_sort novel dinuclear iridium(iii) complex as a g-quadruplex-selective probe for the luminescent switch-on detection of transcription factor hif-1α
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773817/
https://www.ncbi.nlm.nih.gov/pubmed/26932240
http://dx.doi.org/10.1038/srep22458
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