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Periscope: quantitative prediction of soluble protein expression in the periplasm of Escherichia coli

Periplasmic expression of soluble proteins in Escherichia coli not only offers a much-simplified downstream purification process, but also enhances the probability of obtaining correctly folded and biologically active proteins. Different combinations of signal peptides and target proteins lead to di...

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Detalles Bibliográficos
Autores principales: Chang, Catherine Ching Han, Li, Chen, Webb, Geoffrey I., Tey, BengTi, Song, Jiangning, Ramanan, Ramakrishnan Nagasundara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773868/
https://www.ncbi.nlm.nih.gov/pubmed/26931649
http://dx.doi.org/10.1038/srep21844