Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin

Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from...

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Detalles Bibliográficos
Autores principales: Mihara, Emiko, Hirai, Hidenori, Yamamoto, Hideki, Tamura-Kawakami, Keiko, Matano, Mami, Kikuchi, Akira, Sato, Toshiro, Takagi, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775226/
https://www.ncbi.nlm.nih.gov/pubmed/26902720
http://dx.doi.org/10.7554/eLife.11621

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775226/
https://www.ncbi.nlm.nih.gov/pubmed/26902720
http://dx.doi.org/10.7554/eLife.11621

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