Detection of siRNA-mediated target mRNA cleavage activities in human cells by a novel stem-loop array RT-PCR analysis

The small interfering RNA (siRNA)-mediated target mRNA cleavage activity generates cleaved mRNA fragments with varied termini, which creates major technical challenges for the accurate and efficient detection and verification of cleavage sites on target mRNAs. Here we used a sensitive stem-loop arra...

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Detalles Bibliográficos
Autores principales: Lin, Jing, Xu, Kai, Roth, Jack A., Ji, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776327/
https://www.ncbi.nlm.nih.gov/pubmed/26949742
http://dx.doi.org/10.1016/j.bbrep.2016.02.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776327/
https://www.ncbi.nlm.nih.gov/pubmed/26949742
http://dx.doi.org/10.1016/j.bbrep.2016.02.012

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