Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA. To examine the most ac...

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Detalles Bibliográficos
Autores principales: Nakayama, Yuki, Yamaguchi, Hiromi, Einaga, Naoki, Esumi, Mariko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777359/
https://www.ncbi.nlm.nih.gov/pubmed/26937682
http://dx.doi.org/10.1371/journal.pone.0150528

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777359/
https://www.ncbi.nlm.nih.gov/pubmed/26937682
http://dx.doi.org/10.1371/journal.pone.0150528

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