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Pitfalls of DNA Quantification Using DNA-Binding Fluorescent Dyes and Suggested Solutions
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA. To examine the most ac...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777359/ https://www.ncbi.nlm.nih.gov/pubmed/26937682 http://dx.doi.org/10.1371/journal.pone.0150528 |