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Characterization of a Chitosanase from Jelly Fig (Ficus awkeotsang Makino) Latex and Its Application in the Production of Water-Soluble Low Molecular Weight Chitosans
A chitosanase was purified from jelly fig latex by ammonium sulfate fractionation (50–80% saturation) and three successive column chromatography steps. The purified enzyme was almost homogeneous, as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gel activity staining. The molecu...
Autores principales: | Chang, Chen-Tien, Lin, Yen-Lu, Lu, Shu-Wei, Huang, Chun-Wei, Wang, Yu-Ting, Chung, Yun-Chin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4777521/ https://www.ncbi.nlm.nih.gov/pubmed/26938062 http://dx.doi.org/10.1371/journal.pone.0150490 |
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