Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis in patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated hemopha...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778860/ https://www.ncbi.nlm.nih.gov/pubmed/26942577 http://dx.doi.org/10.1371/journal.pntd.0004505 |
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author | Morimoto, Ayako Omachi, Satoko Osada, Yasutaka Chambers, James K. Uchida, Kazuyuki Sanjoba, Chizu Matsumoto, Yoshitsugu Goto, Yasuyuki |
author_facet | Morimoto, Ayako Omachi, Satoko Osada, Yasutaka Chambers, James K. Uchida, Kazuyuki Sanjoba, Chizu Matsumoto, Yoshitsugu Goto, Yasuyuki |
author_sort | Morimoto, Ayako |
collection | PubMed |
description | Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis in patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated hemophagocytosis remained elusive due to a lack of appropriate animal models. Here, we have established a mouse model of VL with hemophagocytosis. At 24 weeks after infection with 1 x 10(7) Leishmania donovani promastigotes, BALB/cA mice exhibited splenomegaly with an average tissue weight per body weight of 2.96%. In the tissues, 28.6% of macrophages contained phagocytosed erythrocytes. All of the hemophagocytosing macrophages were parasitized by L. donovani, and higher levels of hemophagocytosis was observed in heavily infected cells. Furthermore, more than half of these hemophagocytes had two or more macrophage-derived nuclei, whereas only 15.0% of splenic macrophages were bi- or multi-nuclear. These results suggest that direct infection by L. donovani causes hyper-activation of host macrophages to engulf blood cells. To our knowledge, this is the first report on hemophagocytosis in experimental Leishmania infections and may be useful for further understanding of the pathogenesis. |
format | Online Article Text |
id | pubmed-4778860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47788602016-03-23 Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani Morimoto, Ayako Omachi, Satoko Osada, Yasutaka Chambers, James K. Uchida, Kazuyuki Sanjoba, Chizu Matsumoto, Yoshitsugu Goto, Yasuyuki PLoS Negl Trop Dis Research Article Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis in patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated hemophagocytosis remained elusive due to a lack of appropriate animal models. Here, we have established a mouse model of VL with hemophagocytosis. At 24 weeks after infection with 1 x 10(7) Leishmania donovani promastigotes, BALB/cA mice exhibited splenomegaly with an average tissue weight per body weight of 2.96%. In the tissues, 28.6% of macrophages contained phagocytosed erythrocytes. All of the hemophagocytosing macrophages were parasitized by L. donovani, and higher levels of hemophagocytosis was observed in heavily infected cells. Furthermore, more than half of these hemophagocytes had two or more macrophage-derived nuclei, whereas only 15.0% of splenic macrophages were bi- or multi-nuclear. These results suggest that direct infection by L. donovani causes hyper-activation of host macrophages to engulf blood cells. To our knowledge, this is the first report on hemophagocytosis in experimental Leishmania infections and may be useful for further understanding of the pathogenesis. Public Library of Science 2016-03-04 /pmc/articles/PMC4778860/ /pubmed/26942577 http://dx.doi.org/10.1371/journal.pntd.0004505 Text en © 2016 Morimoto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Morimoto, Ayako Omachi, Satoko Osada, Yasutaka Chambers, James K. Uchida, Kazuyuki Sanjoba, Chizu Matsumoto, Yoshitsugu Goto, Yasuyuki Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani |
title | Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani |
title_full | Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani |
title_fullStr | Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani |
title_full_unstemmed | Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani |
title_short | Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani |
title_sort | hemophagocytosis in experimental visceral leishmaniasis by leishmania donovani |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778860/ https://www.ncbi.nlm.nih.gov/pubmed/26942577 http://dx.doi.org/10.1371/journal.pntd.0004505 |
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