Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani

Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis in patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated hemopha...

Descripción completa

Detalles Bibliográficos
Autores principales: Morimoto, Ayako, Omachi, Satoko, Osada, Yasutaka, Chambers, James K., Uchida, Kazuyuki, Sanjoba, Chizu, Matsumoto, Yoshitsugu, Goto, Yasuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778860/
https://www.ncbi.nlm.nih.gov/pubmed/26942577
http://dx.doi.org/10.1371/journal.pntd.0004505
_version_ 1782419540136689664
author Morimoto, Ayako
Omachi, Satoko
Osada, Yasutaka
Chambers, James K.
Uchida, Kazuyuki
Sanjoba, Chizu
Matsumoto, Yoshitsugu
Goto, Yasuyuki
author_facet Morimoto, Ayako
Omachi, Satoko
Osada, Yasutaka
Chambers, James K.
Uchida, Kazuyuki
Sanjoba, Chizu
Matsumoto, Yoshitsugu
Goto, Yasuyuki
author_sort Morimoto, Ayako
collection PubMed
description Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis in patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated hemophagocytosis remained elusive due to a lack of appropriate animal models. Here, we have established a mouse model of VL with hemophagocytosis. At 24 weeks after infection with 1 x 10(7) Leishmania donovani promastigotes, BALB/cA mice exhibited splenomegaly with an average tissue weight per body weight of 2.96%. In the tissues, 28.6% of macrophages contained phagocytosed erythrocytes. All of the hemophagocytosing macrophages were parasitized by L. donovani, and higher levels of hemophagocytosis was observed in heavily infected cells. Furthermore, more than half of these hemophagocytes had two or more macrophage-derived nuclei, whereas only 15.0% of splenic macrophages were bi- or multi-nuclear. These results suggest that direct infection by L. donovani causes hyper-activation of host macrophages to engulf blood cells. To our knowledge, this is the first report on hemophagocytosis in experimental Leishmania infections and may be useful for further understanding of the pathogenesis.
format Online
Article
Text
id pubmed-4778860
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47788602016-03-23 Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani Morimoto, Ayako Omachi, Satoko Osada, Yasutaka Chambers, James K. Uchida, Kazuyuki Sanjoba, Chizu Matsumoto, Yoshitsugu Goto, Yasuyuki PLoS Negl Trop Dis Research Article Hemophagocytosis is a phenomenon in which macrophages phagocytose blood cells. There are reports on up-regulated hemophagocytosis in patients with infectious diseases including typhoid fever, tuberculosis, influenza and visceral leishmaniasis (VL). However, mechanisms of infection-associated hemophagocytosis remained elusive due to a lack of appropriate animal models. Here, we have established a mouse model of VL with hemophagocytosis. At 24 weeks after infection with 1 x 10(7) Leishmania donovani promastigotes, BALB/cA mice exhibited splenomegaly with an average tissue weight per body weight of 2.96%. In the tissues, 28.6% of macrophages contained phagocytosed erythrocytes. All of the hemophagocytosing macrophages were parasitized by L. donovani, and higher levels of hemophagocytosis was observed in heavily infected cells. Furthermore, more than half of these hemophagocytes had two or more macrophage-derived nuclei, whereas only 15.0% of splenic macrophages were bi- or multi-nuclear. These results suggest that direct infection by L. donovani causes hyper-activation of host macrophages to engulf blood cells. To our knowledge, this is the first report on hemophagocytosis in experimental Leishmania infections and may be useful for further understanding of the pathogenesis. Public Library of Science 2016-03-04 /pmc/articles/PMC4778860/ /pubmed/26942577 http://dx.doi.org/10.1371/journal.pntd.0004505 Text en © 2016 Morimoto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Morimoto, Ayako
Omachi, Satoko
Osada, Yasutaka
Chambers, James K.
Uchida, Kazuyuki
Sanjoba, Chizu
Matsumoto, Yoshitsugu
Goto, Yasuyuki
Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
title Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
title_full Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
title_fullStr Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
title_full_unstemmed Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
title_short Hemophagocytosis in Experimental Visceral Leishmaniasis by Leishmania donovani
title_sort hemophagocytosis in experimental visceral leishmaniasis by leishmania donovani
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778860/
https://www.ncbi.nlm.nih.gov/pubmed/26942577
http://dx.doi.org/10.1371/journal.pntd.0004505
work_keys_str_mv AT morimotoayako hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT omachisatoko hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT osadayasutaka hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT chambersjamesk hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT uchidakazuyuki hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT sanjobachizu hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT matsumotoyoshitsugu hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani
AT gotoyasuyuki hemophagocytosisinexperimentalvisceralleishmaniasisbyleishmaniadonovani