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The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice

Kurozu is a traditional Japanese rice vinegar. During fermentation and aging of the Kurozu liquid in an earthenware jar over 1 year, a solid residue called Kurozu Moromi is produced. In the present study, we evaluated whether concentrated Kurozu or Kurozu Moromi could ameliorate cognitive dysfunctio...

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Autores principales: Kanouchi, Hiroaki, Kakimoto, Toshiaki, Nakano, Hideya, Suzuki, Masahiro, Nakai, Yuji, Shiozaki, Kazuhiro, Akikoka, Kohei, Otomaru, Konosuke, Nagano, Masanobu, Matsumoto, Mitsuharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778952/
https://www.ncbi.nlm.nih.gov/pubmed/26943920
http://dx.doi.org/10.1371/journal.pone.0150796
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author Kanouchi, Hiroaki
Kakimoto, Toshiaki
Nakano, Hideya
Suzuki, Masahiro
Nakai, Yuji
Shiozaki, Kazuhiro
Akikoka, Kohei
Otomaru, Konosuke
Nagano, Masanobu
Matsumoto, Mitsuharu
author_facet Kanouchi, Hiroaki
Kakimoto, Toshiaki
Nakano, Hideya
Suzuki, Masahiro
Nakai, Yuji
Shiozaki, Kazuhiro
Akikoka, Kohei
Otomaru, Konosuke
Nagano, Masanobu
Matsumoto, Mitsuharu
author_sort Kanouchi, Hiroaki
collection PubMed
description Kurozu is a traditional Japanese rice vinegar. During fermentation and aging of the Kurozu liquid in an earthenware jar over 1 year, a solid residue called Kurozu Moromi is produced. In the present study, we evaluated whether concentrated Kurozu or Kurozu Moromi could ameliorate cognitive dysfunction in the senescence-accelerated P8 mouse. Senescence-accelerated P8 mice were fed 0.25% (w/w) concentrated Kurozu or 0.5% (w/w) Kurozu Moromi for 4 or 25 weeks. Kurozu suppressed cognitive dysfunction and amyloid accumulation in the brain, while Kurozu Moromi showed a tendency to ameliorate cognitive dysfunction, but the effect was not significant. We hypothesize that concentrated Kurozu has an antioxidant effect; however, the level of lipid peroxidation in the brain did not differ in senescence-accelerated P8 mice. DNA microarray analysis indicated that concentrated Kurozu increased HSPA1A mRNA expression, a protein that prevents protein misfolding and aggregation. The increase in HSPA1A expression by Kurozu was confirmed using quantitative real-time PCR and immunoblotting methods. The suppression of amyloid accumulation by concentrated Kurozu may be associated with HSPA1A induction. However, concentrated Kurozu could not increase HSPA1A expression in mouse primary neurons, suggesting it may not directly affect neurons.
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spelling pubmed-47789522016-03-23 The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice Kanouchi, Hiroaki Kakimoto, Toshiaki Nakano, Hideya Suzuki, Masahiro Nakai, Yuji Shiozaki, Kazuhiro Akikoka, Kohei Otomaru, Konosuke Nagano, Masanobu Matsumoto, Mitsuharu PLoS One Research Article Kurozu is a traditional Japanese rice vinegar. During fermentation and aging of the Kurozu liquid in an earthenware jar over 1 year, a solid residue called Kurozu Moromi is produced. In the present study, we evaluated whether concentrated Kurozu or Kurozu Moromi could ameliorate cognitive dysfunction in the senescence-accelerated P8 mouse. Senescence-accelerated P8 mice were fed 0.25% (w/w) concentrated Kurozu or 0.5% (w/w) Kurozu Moromi for 4 or 25 weeks. Kurozu suppressed cognitive dysfunction and amyloid accumulation in the brain, while Kurozu Moromi showed a tendency to ameliorate cognitive dysfunction, but the effect was not significant. We hypothesize that concentrated Kurozu has an antioxidant effect; however, the level of lipid peroxidation in the brain did not differ in senescence-accelerated P8 mice. DNA microarray analysis indicated that concentrated Kurozu increased HSPA1A mRNA expression, a protein that prevents protein misfolding and aggregation. The increase in HSPA1A expression by Kurozu was confirmed using quantitative real-time PCR and immunoblotting methods. The suppression of amyloid accumulation by concentrated Kurozu may be associated with HSPA1A induction. However, concentrated Kurozu could not increase HSPA1A expression in mouse primary neurons, suggesting it may not directly affect neurons. Public Library of Science 2016-03-04 /pmc/articles/PMC4778952/ /pubmed/26943920 http://dx.doi.org/10.1371/journal.pone.0150796 Text en © 2016 Kanouchi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kanouchi, Hiroaki
Kakimoto, Toshiaki
Nakano, Hideya
Suzuki, Masahiro
Nakai, Yuji
Shiozaki, Kazuhiro
Akikoka, Kohei
Otomaru, Konosuke
Nagano, Masanobu
Matsumoto, Mitsuharu
The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice
title The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice
title_full The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice
title_fullStr The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice
title_full_unstemmed The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice
title_short The Brewed Rice Vinegar Kurozu Increases HSPA1A Expression and Ameliorates Cognitive Dysfunction in Aged P8 Mice
title_sort brewed rice vinegar kurozu increases hspa1a expression and ameliorates cognitive dysfunction in aged p8 mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778952/
https://www.ncbi.nlm.nih.gov/pubmed/26943920
http://dx.doi.org/10.1371/journal.pone.0150796
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