Cargando…

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Genome-editing of human pluripotent stem cells (hPSCs) provides a genetically controlled and clinically relevant platform from which to understand human development and investigate the pathophysiology of disease. By employing site-specific nucleases (SSNs) for genome editing, the rapid derivation of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Blair, John D., Bateup, Helen S., Hockemeyer, Dirk F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2016
Materias:	Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781717/ https://www.ncbi.nlm.nih.gov/pubmed/26863600 http://dx.doi.org/10.3791/53583

Ejemplares similares

Genetically-engineered human cortical spheroid models of Tuberous Sclerosis
por: Blair, John D., et al.
Publicado: (2018)

Highly efficient generation of isogenic pluripotent stem cell models using prime editing
por: Li, Hanqin, et al.
Publicado: (2022)

Fast-Track and Integration-Free Method of Genome Editing by CRISPR/Cas9 in Murine Pluripotent Stem Cells
por: Mamun, Md Mahfuz Al, et al.
Publicado: (2022)

Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease
por: Qi, Tao, et al.
Publicado: (2020)

Leukemia Inhibitory Factor (LIF)-dependent, Pluripotent Stem Cells Established from Inner Cell Mass of Porcine Embryos
por: Telugu, Bhanu Prakash V. L., et al.
Publicado: (2011)

Pathological modeling of glycogen storage disease type III with CRISPR/Cas9 edited human pluripotent stem cells
por: Rossiaud, Lucille, et al.
Publicado: (2023)

Isolation of human pluripotent stem cell-derived sensory neuron subtypes by immunopanning
por: Saito-Diaz, Kenyi, et al.
Publicado: (2023)

HIPPO signaling resolves embryonic cell fate conflicts during establishment of pluripotency in vivo
por: Frum, Tristan, et al.
Publicado: (2018)

Human Induced Pluripotent Stem Cell-Derived TDP-43 Mutant Neurons Exhibit Consistent Functional Phenotypes Across Multiple Gene Edited Lines Despite Transcriptomic and Splicing Discrepancies
por: Smith, Alec S. T., et al.
Publicado: (2021)

Reprogramming of spermatogonial stem cells into pluripotent stem cells in the spheroidal state
por: Lee, Yukyeong, et al.
Publicado: (2019)

A Benchmark Side-by-Side Comparison of Two Well-Established Protocols for in vitro Hematopoietic Differentiation From Human Pluripotent Stem Cells
por: Gutierrez-Agüera, Francisco, et al.
Publicado: (2021)

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
por: Wang, Yu, et al.
Publicado: (2017)

Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells
por: Gumber, Diana, et al.
Publicado: (2020)

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
por: Frega, Monica, et al.
Publicado: (2017)

Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
por: Bauwens, Celine L., et al.
Publicado: (2016)

Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes
por: Selvaraj, Sridhar, et al.
Publicado: (2019)

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
por: Stevanato, Lara, et al.
Publicado: (2015)

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
por: Krefft, Olivia, et al.
Publicado: (2018)

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
por: Stover, Alexander E., et al.
Publicado: (2016)

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
por: Pistollato, Francesca, et al.
Publicado: (2017)

A role for TGFβ signalling in medium spiny neuron differentiation of human pluripotent stem cells
por: Fjodorova, Marija, et al.
Publicado: (2020)

Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells
por: Ng, Wai Hoe, et al.
Publicado: (2022)

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
por: Meseguer-Ripolles, Jose, et al.
Publicado: (2018)

Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference
por: Libby, Ashley RG, et al.
Publicado: (2018)

Differentiation of human induced pluripotent stem cells into cortical neural stem cells
por: Neaverson, Alexandra, et al.
Publicado: (2023)

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
por: Parsons, Xuejun H., et al.
Publicado: (2011)

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
por: Foltz, Leah P., et al.
Publicado: (2017)

Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells
por: Seita, Yasunari, et al.
Publicado: (2023)

Utilization of Human Induced Pluripotent Stem Cells for Cardiac Repair
por: Fan, Chengming, et al.
Publicado: (2020)

Uncovering Inherited Cardiomyopathy With Human Induced Pluripotent Stem Cells
por: Jiang, Xue, et al.
Publicado: (2021)

Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells
por: Kumar, Nathan, et al.
Publicado: (2015)

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
por: Kishino, Yoshikazu, et al.
Publicado: (2015)

Energy Metabolism Regulates Stem Cell Pluripotency
por: Tsogtbaatar, Enkhtuul, et al.
Publicado: (2020)

Editorial: Reviews in induced pluripotent stem cells
por: Dottori, Mirella, et al.
Publicado: (2023)

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
por: Sala, Luca, et al.
Publicado: (2017)

Wnt signaling mediates acquisition of blood–brain barrier properties in naïve endothelium derived from human pluripotent stem cells
por: Gastfriend, Benjamin D, et al.
Publicado: (2021)

An Efficient Protocol for Single-Cell Cloning Human Pluripotent Stem Cells
por: Singh, Amar M.
Publicado: (2019)

Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
por: Sun, Xuetao, et al.
Publicado: (2017)

Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids
por: Chang, Mingyang, et al.
Publicado: (2021)

Editorial: Generation of Functional tissues from human pluripotent stem cells
por: Guo, Dongsheng, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_puoae8mmiaf9inhtigfvn954g4