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Sanger Sequencing for BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del Mutation Screen on Pap Smear Cytology Samples

Three sets of polymerase chain reaction (PCR) primers were designed for heminested PCR amplification of the target DNA fragments in the human genome which include the site of BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del respectively, to prepare the templates for direct Sanger sequencing scr...

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Detalles Bibliográficos
Autores principales: Lee, Sin Hang, Zhou, Shaoxia, Zhou, Tianjun, Hong, Guofan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4783961/
https://www.ncbi.nlm.nih.gov/pubmed/26867194
http://dx.doi.org/10.3390/ijms17020229