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Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization
ABSTRACT: Bioorthogonal ligations have emerged as highly versatile chemical tools for biomedical research. The exceptionally fast reaction between 1,2,4,5-tetrazines and trans-cyclooctenes (TCOs), also known as tetrazine ligation, is frequently used in this regard. Growing numbers of applications fo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785212/ https://www.ncbi.nlm.nih.gov/pubmed/27069284 http://dx.doi.org/10.1007/s00706-016-1668-z |
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author | Svatunek, Dennis Denk, Christoph Rosecker, Veronika Sohr, Barbara Hametner, Christian Allmaier, Günter Fröhlich, Johannes Mikula, Hannes |
author_facet | Svatunek, Dennis Denk, Christoph Rosecker, Veronika Sohr, Barbara Hametner, Christian Allmaier, Günter Fröhlich, Johannes Mikula, Hannes |
author_sort | Svatunek, Dennis |
collection | PubMed |
description | ABSTRACT: Bioorthogonal ligations have emerged as highly versatile chemical tools for biomedical research. The exceptionally fast reaction between 1,2,4,5-tetrazines and trans-cyclooctenes (TCOs), also known as tetrazine ligation, is frequently used in this regard. Growing numbers of applications for the tetrazine ligation led to an increased demand for TCO compounds, whose commercial availability is still very limited. Reported photochemical procedures for the preparation of TCOs using flow chemistry are straightforward and high yielding but require expensive equipment. Within this contribution, we present the construction and characterization of a low-cost flow photoreactor assembled from readily accessible components. Syntheses of all commonly used trans-cyclooctene derivatives were successfully carried out using the described system. We are convinced that the presented system for photoisomerization will promote access to bioorthogonally reactive TCO derivatives. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00706-016-1668-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4785212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-47852122016-04-09 Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization Svatunek, Dennis Denk, Christoph Rosecker, Veronika Sohr, Barbara Hametner, Christian Allmaier, Günter Fröhlich, Johannes Mikula, Hannes Monatsh Chem Original Paper ABSTRACT: Bioorthogonal ligations have emerged as highly versatile chemical tools for biomedical research. The exceptionally fast reaction between 1,2,4,5-tetrazines and trans-cyclooctenes (TCOs), also known as tetrazine ligation, is frequently used in this regard. Growing numbers of applications for the tetrazine ligation led to an increased demand for TCO compounds, whose commercial availability is still very limited. Reported photochemical procedures for the preparation of TCOs using flow chemistry are straightforward and high yielding but require expensive equipment. Within this contribution, we present the construction and characterization of a low-cost flow photoreactor assembled from readily accessible components. Syntheses of all commonly used trans-cyclooctene derivatives were successfully carried out using the described system. We are convinced that the presented system for photoisomerization will promote access to bioorthogonally reactive TCO derivatives. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00706-016-1668-z) contains supplementary material, which is available to authorized users. Springer Vienna 2016-02-22 2016 /pmc/articles/PMC4785212/ /pubmed/27069284 http://dx.doi.org/10.1007/s00706-016-1668-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Svatunek, Dennis Denk, Christoph Rosecker, Veronika Sohr, Barbara Hametner, Christian Allmaier, Günter Fröhlich, Johannes Mikula, Hannes Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
title | Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
title_full | Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
title_fullStr | Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
title_full_unstemmed | Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
title_short | Efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
title_sort | efficient low-cost preparation of trans-cyclooctenes using a simplified flow setup for photoisomerization |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785212/ https://www.ncbi.nlm.nih.gov/pubmed/27069284 http://dx.doi.org/10.1007/s00706-016-1668-z |
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