A new method to prevent carry-over contaminations in two-step PCR NGS library preparations

Two-step PCR procedures are an efficient and well established way to generate amplicon libraries for NGS sequencing. However, there is a high risk of cross-contamination by carry-over of amplicons from first to second amplification rounds, potentially leading to severe misinterpretation of results....

Descripción completa

Detalles Bibliográficos
Autores principales: Seitz, Volkhard, Schaper, Sigrid, Dröge, Anja, Lenze, Dido, Hummel, Michael, Hennig, Steffen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787772/
https://www.ncbi.nlm.nih.gov/pubmed/26152304
http://dx.doi.org/10.1093/nar/gkv694

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787772/
https://www.ncbi.nlm.nih.gov/pubmed/26152304
http://dx.doi.org/10.1093/nar/gkv694

Ejemplares similares