Hairpins under tension: RNA versus DNA

We use optical tweezers to control the folding and unfolding of individual DNA and RNA hairpins by force. Four hairpin molecules are studied in comparison: two DNA and two RNA ones. We observe that the conformational dynamics is slower for the RNA hairpins than for their DNA counterparts. Our result...

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Detalles Bibliográficos
Autores principales: Bercy, Mathilde, Bockelmann, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787782/
https://www.ncbi.nlm.nih.gov/pubmed/26323319
http://dx.doi.org/10.1093/nar/gkv860
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author Bercy, Mathilde
Bockelmann, Ulrich
author_facet Bercy, Mathilde
Bockelmann, Ulrich
author_sort Bercy, Mathilde
collection PubMed
description We use optical tweezers to control the folding and unfolding of individual DNA and RNA hairpins by force. Four hairpin molecules are studied in comparison: two DNA and two RNA ones. We observe that the conformational dynamics is slower for the RNA hairpins than for their DNA counterparts. Our results indicate that structures made of RNA are dynamically more stable. This difference might contribute to the fact that DNA and RNA play fundamentally different biological roles in spite of chemical similarity.
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spelling pubmed-47877822016-03-14 Hairpins under tension: RNA versus DNA Bercy, Mathilde Bockelmann, Ulrich Nucleic Acids Res RNA We use optical tweezers to control the folding and unfolding of individual DNA and RNA hairpins by force. Four hairpin molecules are studied in comparison: two DNA and two RNA ones. We observe that the conformational dynamics is slower for the RNA hairpins than for their DNA counterparts. Our results indicate that structures made of RNA are dynamically more stable. This difference might contribute to the fact that DNA and RNA play fundamentally different biological roles in spite of chemical similarity. Oxford University Press 2015-11-16 2015-08-31 /pmc/articles/PMC4787782/ /pubmed/26323319 http://dx.doi.org/10.1093/nar/gkv860 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Bercy, Mathilde
Bockelmann, Ulrich
Hairpins under tension: RNA versus DNA
title Hairpins under tension: RNA versus DNA
title_full Hairpins under tension: RNA versus DNA
title_fullStr Hairpins under tension: RNA versus DNA
title_full_unstemmed Hairpins under tension: RNA versus DNA
title_short Hairpins under tension: RNA versus DNA
title_sort hairpins under tension: rna versus dna
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787782/
https://www.ncbi.nlm.nih.gov/pubmed/26323319
http://dx.doi.org/10.1093/nar/gkv860
work_keys_str_mv AT bercymathilde hairpinsundertensionrnaversusdna
AT bockelmannulrich hairpinsundertensionrnaversusdna

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