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Nucleotidyl transferase assisted DNA labeling with different click chemistries

Here, we present a simple, modular and efficient strategy that allows the 3′-terminal labeling of DNA, regardless of whether it has been chemically or enzymatically synthesized or isolated from natural sources. We first incorporate a range of modified nucleotides at the 3′-terminus, using terminal d...

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Detalles Bibliográficos
Autores principales: Winz, Marie-Luise, Linder, Eva Christina, André, Timon, Becker, Juliane, Jäschke, Andres
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787804/
https://www.ncbi.nlm.nih.gov/pubmed/26013812
http://dx.doi.org/10.1093/nar/gkv544