Cargando…

Global analysis of RNA cleavage by 5′-hydroxyl RNA sequencing

RNA cleavage by some endoribonucleases and self-cleaving ribozymes produces RNA fragments with 5′-hydroxyl (5′-OH) and 2′,3′-cyclic phosphate termini. To identify 5′-OH RNA fragments produced by these cleavage events, we exploited the unique ligation mechanism of Escherichia coli RtcB RNA ligase to...

Descripción completa

Detalles Bibliográficos
Autores principales: Peach, Sally E., York, Kerri, Hesselberth, Jay R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4787814/
https://www.ncbi.nlm.nih.gov/pubmed/26001965
http://dx.doi.org/10.1093/nar/gkv536